Comparative efficiency of external drugs with antibacterial action for experimental wound treatment
Abstract
The aim of the study was to determine the rate of wound
healing and compare the effectiveness of registered external antibacterial
wound healing agents.
Material and methods. The study was performed on old Wistar rats (body
weight Me=425 g), which under non-sterile conditions inflicted wounds of a
given size (121.9±2.1 mm2). We studied 4 groups of wounds: 2
without treatment (Control_0 - intact; Control_ps - injected physiological
solution on the day of modeling), as well as 2 using external antibacterial
drugs (silver sulfathiazole (SS), chloramphenicol with
dioxomethyltetrahydropyrimidine (CD). At 0, 1, 3, 5, 7, 14 days, the
dynamic area of the wounds, their lobe change relative to the initial
sizes, the dynamic thermometry of the wounds. Histological examination of
the wounds was performed on days 3, 7, 14.
Results. Within 2 weeks, the area of intact wounds was reduced
by 6 times, the inflammatory period lasted up to 3 days, positive changes were
recorded from 5 days. In the Control_ps group, the period of wound expansion
lasted up to 7 days, significant healing was recorded on day 14, when
the wound area regressed 4.5 times relative to the initial values. In the
SS and CD groups, a significant increase in wounds was established on days
1 and 3 with a return to the initial level on the 5th day, wound
reduction was recorded from 7 days in the SS group, and DX on the 14th day.
By the 14th day, the area of wounds of the SS group
decreased on average by 88%, CD - 85%, which is comparable with the
Control_0 group (83%). Morphological analysis showed a tendency to impaired
wound healing up to 7 days with the use of CD and SS groups.
Conclusion. The expediency of wide early use of tested
antimicrobial ointments for the treatment of wounds that do not have signs of
inflammation and bacterial contamination on the first day has not been
proven.
Keywords:skin wounds, wound healing, wound area, regeneration rate, silver sulfathiazole, chloramphenicol with dioxomethyltetrahydro-pyrimidine
Funding. The study is part of the work carried out with the
support of the Fund for Assistance to Small Innovative Enterprises in Science
and Technology (Fund for Assistance to Innovation, contract No. 232GRNTIS5 /
35963 of 08.08.2017).
Conflict of interests. The authors declare no conflict of interests.
Contribution. Conceptualization, preparation of the text of the
article, verification - Stupin V.A.; visualization, statistical processing -
Vasin V.I.; reviewing and editing of the article - Manturova N.E.; formal
analysis - Artyushkova E.B.; data curation - Gladchenko M.P.; methodology
- Ivanov A.V.; editing - Saltykov A.B.; conceptualization, statistical
processing, preparation of the text of the article - Silina E.V.
For citation: Stupin V.A., Vasin V.I., Manturova N.E., Artyushkova
E.B., Gladchenko M.P., Ivanov A.V., Saltykov A.B., Silina E.V. Comparative
efficiency of external drugs with antibacterial action for experimental wound
treatment. Clinical and Experimental Surgery. Petrovsky Journal. 2020; 8
(4): 135-42. DOI: https://doi.org/10.33029/2308-1198-2020-8-4-135-142 (in Russian)
References
1. Baron J.M., Glatz M., Proksch E. Optimal support of wound healing: new insights. Dermatology. 2020; 17: 1-8. DOI: https://doi.org/10.1159/000505291
2. Ribeiro D.M.L., Carvalho Junior A.R., Vale de Macedo G.H.R., Chagas V.L., Silva L.D.S., Cutrim B.D.S., et al. Polysaccharide-based formulations for healing of skin-related wound infections: lessons from animal models and clinical trials. Biomolecules. 2019; 10 (1): E63. DOI: https://doi.org/10.3390/biom10010063
3. Shukla S.K., Sharma A.K., Gupta V., Yashavard-dhan M.H. Pharmacological control of inflammation in wound healing. J Tissue Viability. 2019; 28 (4): 218-22. DOI: https://doi.org/10.1016/jjtv.2019.09.002
4. Silina E.V., Khokhlov N.V., Stupin V.A., Mantu-rova N.E., Vasin V.I., Velikanov E.V., et al. Multicomponent polysaccharide essential formula of wound healing medicines enriched with fibroblast growth factor. Int J Biomed. 2019; 9 (3): 247-50. DOI: https://doi.org/10.21103/Article9(3)_OA12
5. Stupin V.A., Silina E.V., Gorsky V.A., Goryunov S.V., Zhidkikh S.Yu., Komarov A.N., et al. Efficacy and safety of collagen biomaterial local application in complex treatment of the diabetic foot syndrome (final results of the multicenter randomised study). Khirurgiya. Zhur-nal imeni N.I. Pirogova [Surgery. The Journal Named after N.I. Pirogov]. 2018; (6): 91-100. DOI: https://doi.org/10.17116/hirurgia2018691-100 (in Russian)
6. Gareev I.F., Beylerli O.A.O. Combined cranio-abdominal injury. Clinical epidemiology. Rossiyskiy ney-rokhirurgicheskiy zhurnal imeni professora A.L. Polenova [Russian Neurosurgical Journal Named after Professor A.L. Polenov]. 2018; 10 (S): 25-6. (in Russian)
7. Pyankova A.I., Fattakhov T.A. Lost years of healthy life as a result of traffic accidents in Russia. Profilakticheskaya meditsina [Preventive Medicine]. 2017; 20 (5): 30-6. DOI: https://doi.org/10.17116/profmed201720530-36 (in Russian)
8. Wang Y., Beekman J., Hew J., Jackson S., Issler-Fisher A.C., Parungao R., et al. Burn injury: challenges and advances in burn wound healing, infection, pain and scarring. Adv Drug Deliv Rev. 2018; 123: 3-17. DOI: https://doi.org/10.1016/j.addr.2017.09.018
9. Rosberg H.E., Dahlin L.B. An increasing number of hand injuries in an elderly population - a retrospective study over a 30-year period. BMC Geriatr. 2018; 18 (1): 68. DOI: https://doi.org/10.1186/s12877-018-0758-7
10. Dibirov M.D., Magdiev A.Kh. Treatment of venous trophic ulcers in old age. Flebologiya [Flebology]. 2016; 10 (4): 224-8. DOI: https://doi.org/10.17116/flebo2016104224-228 (in Russian)
11. Antonopoulos C.N., Lazaris A., Venermo M., Ger-oulakos G. Predictors of wound healing following revascularization for chronic limb-threatening ischemia. Vasc Endovascular Surg. 2019; 53 (8): 649-57. DOI: https://doi.org/10.1177/1538574419868863
12. Han G., Ceilley R. Chronic wound healing: a review of current management and treatments. Adv Ther. 2017; 34 (3): 599-610. DOI: https://doi.org/10.1007/s12325-017-0478-y
13. Stupin V.A., Gabitov R.B., Sinelnikova T.G., Silina E.V. Biological mechanisms of the chronic wound and diabetic foot healing: the role of collagen. Serbian J Exp Clin Res. 2018; 19 (4): 373-82. DOI: https://doi.org/10.2478/SJECR-2018-0077
14. Patent RF na poleznuju model’ №79701/10.01.2009. Bjul. No. 1. Khruslov M.V., Kalutsky P.V., Ivanov A.V., Zyuzya E.V. Ustrojstvo dlja modelirovanija ranevoj poverhnosti zadannyh razmerov u laboratornyh zhivotnyh. URL: https://yandex.ru/patents/doc/RU79701U1_20090110 (date of access March 05, 2020) (in Russian)